![[KO Validated] EHMT2 Rabbit pAb (A1247)](/upload/resize_cache/iblock/e3e/340_340_140cd750bba9870f18aada2478b24840a/s0dijewcr6drw1n0wo9bx2luju5njsnq.jpg "[KO Validated] EHMT2 Rabbit pAb (A1247)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] EHMT2 Rabbit pAb (A1247)
- Описание
- Характеристики
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Overview
Product name [KO Validated] EHMT2 Rabbit pAb Catalog No. A1247 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 877-1176 of human EHMT2 (NP_079532.5). Sequence VGNRAIRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRNCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPELGSLPPVNT Gene ID Swiss Prot Synonyms G9A; BAT8; GAT8; NG36; KMT1C; C6orf30 Calculated MW 132kDa Observed MW 150kDa/170kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IP Recommended dilution - WB 1:500 - 1:2000
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunoprecipitation Positive samples U-937, 293T, Jurkat, MCF7, HepG2 Cellular location Chromosome, Nucleus 
Western blot analysis of extracts of various cell lines, using EHMT2 antibody (A1247) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts from normal (control) and EHMT2 Rabbit pAb knockout (KO) 293T cells, using EHMT2 Rabbit pAb antibody (A1247) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg [KO Validated] EHMT2 antibody (A1247). Western blot was performed from the immunoprecipitate using [KO Validated] EHMT2 antibody (A1247) at a dilution of 1:1000.
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Key applications Western blotting, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 877-1176 of human EHMT2 (NP_079532.5). KO Validated Validated Isotype IgG