![[KO Validated] CD133 Rabbit pAb (A12711)](/upload/resize_cache/iblock/9fa/340_340_140cd750bba9870f18aada2478b24840a/2d5z2qyo0yr5vsb0uaqlqxq214hrxt1h.jpg "[KO Validated] CD133 Rabbit pAb (A12711)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] CD133 Rabbit pAb (A12711)
- Описание
- Характеристики
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Overview
Product name [KO Validated] CD133 Rabbit pAb Catalog No. A12711 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 179-433 of human CD133 (NP_006008.1). Sequence ANHQVRTRIKRSRKLADSNFKDLRTLLNETPEQIKYILAQYNTTKDKAFTDLNSINSVLGGGILDRLRPNIIPVLDEIKSMATAIKETKEALENMNSTLKSLHQQSTQLSSSLTSVKTSLRSSLNDPLCLVHPSSETCNSIRLSLSQLNSNPELRQLPPVDAELDNVNNVLRTDLDGLVQQGYQSLNDIPDRVQRQTTTVVAGIKRVLNSIGSDIDNVTQRLPIQDILSAFSVYVNNTESYIHRNLPTLEEYDSY Gene ID Swiss Prot Synonyms RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061 Calculated MW 97kDa Observed MW 120kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC IP Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Immunoprecipitation Positive samples HCT116 Cellular location Apical cell membrane, Cell projection, Endoplasmic reticulum, Endoplasmic reticulum-Golgi intermediate compartment, Multi-pass membrane protein, cilium, microvillus membrane, photoreceptor outer segment Customer validation WB(Mouse Monocyte Macrophage Leukemia Cells, Mus musculus)
IF(Homo sapiens, Rattus norvegicus)
Western blot analysis of extracts of HCT116 cells, using CD133 antibody (A12711) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts from normal (control) and CD133 Rabbit pAb knockout (KO) HCT116 cells, using CD133 Rabbit pAb antibody (A12711) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunoprecipitation analysis of 200 μg extracts of HT-29 cells using 3 μg CD133 antibody (A12711). Western blot was performed from the immunoprecipitate using CD133 antibody (A12711) at a dilution of 1:1000.
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Key applications Western blotting, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 179-433 of human CD133 (NP_006008.1). KO Validated Validated Isotype IgG