![[KO Validated] Peroxiredoxin 4 (PRDX4) Rabbit pAb (A1486)](/upload/resize_cache/iblock/ea8/340_340_140cd750bba9870f18aada2478b24840a/ciqnpkd51k0ljtk4luw23pvano2hke1l.jpg "[KO Validated] Peroxiredoxin 4 (PRDX4) Rabbit pAb (A1486)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] Peroxiredoxin 4 (PRDX4) Rabbit pAb (A1486)
- Описание
- Характеристики
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Overview
Product name [KO Validated] Peroxiredoxin 4 (PRDX4) Rabbit pAb Catalog No. A1486 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 38-271 of human Peroxiredoxin 4 (Peroxiredoxin 4 (PRDX4)) (NP_006397.1). Sequence WETEERPRTREEECHFYAGGQVYPGEASRVSVADHSLHLSKAKISKPAPYWEGTAVIDGEFKELKLTDYRGKYLVFFFYPLDFTFVCPTEIIAFGDRLEEFRSINTEVVACSVDSQFTHLAWINTPRRQGGLGPIRIPLLSDLTHQISKDYGVYLEDSGHTLRGLFIIDDKGILRQITLNDLPVGRSVDETLRLVQAFQYTDKHGEVCPAGWKPGSETIIPDPAGKLKYFDKLN Gene ID Swiss Prot Synonyms PRX-4; AOE372; AOE37-2; HEL-S-97n Calculated MW 31kDa Observed MW 28kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Positive samples H460, PC-3, HeLa, MCF-7, Mouse testis Cellular location Cytoplasm, Secreted 
Western blot analysis of extracts of various cell lines, using Peroxiredoxin 4 (Peroxiredoxin 4 (PRDX4)) antibody (A1486) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and Peroxiredoxin 4 (Peroxiredoxin 4 (PRDX4)) knockout (KO) 293T cells, using Peroxiredoxin 4 (Peroxiredoxin 4 (PRDX4)) antibody (A1486) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 38-271 of human Peroxiredoxin 4 (Peroxiredoxin 4 (PRDX4)) (NP_006397.1). KO Validated Validated Isotype IgG