![[KO Validated] SETD7 Rabbit pAb (A9985)](/upload/resize_cache/iblock/a53/340_340_140cd750bba9870f18aada2478b24840a/igkmiupzawcaup5rnybfxy9fencv27s7.jpg "[KO Validated] SETD7 Rabbit pAb (A9985)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
[KO Validated] SETD7 Rabbit pAb (A9985)
- Описание
- Характеристики
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Overview
Product name [KO Validated] SETD7 Rabbit pAb Catalog No. A9985 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Enables histone-lysine N-methyltransferase activity and p53 binding activity. Involved in peptidyl-lysine dimethylation and peptidyl-lysine monomethylation. Acts upstream of or within cellular response to DNA damage stimulus and heterochromatin organization. Located in chromosome and nucleolus.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-366 of human SETD7 (NP_085151.1). Sequence MDSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK Gene ID Swiss Prot Synonyms KMT7; SET7; SET9; SET7/9 Calculated MW 41kDa Observed MW 48kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples 293T Cellular location Chromosome, Nucleus Customer validation WB(Human hepatocellular carcinoma cell line, Homo sapiens)
IHC(Rattus norvegicus, Mus musculus)
IF(Rattus norvegicus)
IF(Rattus norvegicus)
Western blot analysis of extracts from normal (control) and SETD7 knockout (KO) 293T cells, using [KO Validated] SETD7 antibody (A9985) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-366 of human SETD7 (NP_085151.1). KO Validated Validated Isotype IgG