![[KO Validated] WSTF Rabbit pAb (A9851)](/upload/resize_cache/iblock/5b5/340_340_140cd750bba9870f18aada2478b24840a/9fqm23azoloheh0lvyaibzl3gwaoy8w7.jpg "[KO Validated] WSTF Rabbit pAb (A9851)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
[KO Validated] WSTF Rabbit pAb (A9851)
- Описание
- Характеристики
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Overview
Product name [KO Validated] WSTF Rabbit pAb Catalog No. A9851 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a member of the bromodomain protein family. The bromodomain is a structural motif characteristic of proteins involved in chromatin-dependent regulation of transcription. This gene is deleted in Williams-Beuren syndrome, a developmental disorder caused by deletion of multiple genes at 7q11.23.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human WSTF (NP_115784.1). Sequence MAPLLGRKPFPLVKPLPGEEPLFTIPHTQEAFRTREEYEARLERYSERIWTCKSTGSSQLTHKEAWEEEQEVAELLKEEFPAWYEKLVLEMVHHNTASLEKLVDTAWLEIMTKYAVGEECDFEVGKEKMLKVKIVKIHPLEKVDEEATEKKSDGACDSPSSDKENSSQIAQDHQKKETVVKEDEGRRESINDRARRSPRKLPTSLKKGERKWAPPKFLPHKYDVKLQNEDKIISNVPADSLIRTERPPNKEIVRYFIRHN Gene ID Swiss Prot Synonyms WSTF; WBSCR9; WBSCR10 Calculated MW 171kDa Observed MW 190kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:2000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples SW480, BT-474, SKOV3, HeLa, HepG2, Mouse thymus Cellular location Nucleus Customer validation WB(Homo sapiens)
Western blot analysis of extracts of various cell lines, using WSTF antibody (A9851) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Western blot analysis of extracts from normal (control) and WSTF knockout (KO) HeLa cells, using WSTF antibody (A9851) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded rat brain using WSTF antibody (A9851) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse brain using WSTF antibody (A9851) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human WSTF (NP_115784.1). KO Validated Validated Isotype IgG