![[KO Validated] APEX1/APE1 Rabbit pAb (A2587)](/upload/resize_cache/iblock/255/340_340_140cd750bba9870f18aada2478b24840a/olyxvwja3eqnll9mto6bk4x66g6kkqhc.jpg "[KO Validated] APEX1/APE1 Rabbit pAb (A2587)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
[KO Validated] APEX1/APE1 Rabbit pAb (A2587)
- Описание
- Характеристики
-
Overview
Product name [KO Validated] APEX1/APE1 Rabbit pAb Catalog No. A2587 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The APEX gene encodes the major AP endonuclease in human cells. It encodes the APEX endonuclease, a DNA repair enzyme with apurinic/apyrimidinic (AP) activity. Such AP activity sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. The AP sites are the most frequent pre-mutagenic lesions that can prevent normal DNA replication. Splice variants have been found for this gene; all encode the same protein. Disruptions in the biological functions related to APEX are associated with many various malignancies and neurodegenerative diseases.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human APEX1/APE1 (NP_542380.1). Sequence MPKRGKKGAVAEDGDELRTEPEAKKSKTAAKKNDKEAAGEGPALYEDPPDQKTSPSGKPATLKICSWNVDGLRAWIKKKGLDWVKEEAPDILCLQETKCSENKLPAELQELPGLSHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIGDEEHDQEGRVIVAEFDSFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLADSFRHLYPNTPYAYTFWTYMMNARSKNVGWRLDYFLLSHSLLPALCDSKIRSKALGSDHCPITLYLAL Gene ID Swiss Prot Synonyms APE; APX; APE1; APEN; APEX; HAP1; REF1 Calculated MW 36kDa Observed MW 39kDa Applications
Reactivity Human Tested applications WB IF/ICC IP Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:100
- IP 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Immunoprecipitation Positive samples HeLa, HepG2 Cellular location Cytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus, Nucleus speckle, nucleolus Customer validation WB(Homo sapiens)
Western blot analysis of extracts of various cell lines, using APEX1/APE1 antibody (A2587).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and APEX1/APE1 knockout (KO) 293T cells, using APEX1/APE1 antibody (A2587) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunofluorescence analysis of HeLa cells using APEX1/APE1 antibody (A2587). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A549 cells using APEX1/APE1 antibody (A2587).
Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 1 μg APEX1/APE1 antibody (A2587). Western blot was performed from the immunoprecipitate using APEX1/APE1 antibody (A2587) at a dilution of 1:1000.
-
Key applications Western blotting, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human APEX1/APE1 (NP_542380.1). KO Validated Validated Isotype IgG