быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
TRMT2A/HTF9C Rabbit mAb (A0136)
- Описание
- Характеристики
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Overview
Product name TRMT2A/HTF9C Rabbit mAb Catalog No. A0136 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2498 Background
The protein encoded by this gene is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers. Several transcript variants encoding two different isoforms have been found for this gene.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TRMT2A/HTF9C (Q8IZ69). Sequence MSENLDNEGPKPMESCGQESSSALSCPTVSVPPAAPAALEEVEKEGAGAATGPGPQPGLYSYIRDDLFTSEIFKLELQNVPRHASFSDVRRFLGRFGLQP Gene ID Swiss Prot Synonyms HTF9C Calculated MW 69kDa Observed MW 75kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples MCF7, K-562 Cellular location cytosol 
Western blot analysis of K-562, using TRMT2A/HTF9C Rabbit mAb (A0136) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of MCF7, using TRMT2A/HTF9C Rabbit mAb (A0136) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TRMT2A/HTF9C (Q8IZ69). Isotype IgG