быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
UBE4B Rabbit mAb (A0419)
- Описание
- Характеристики
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Overview
Product name UBE4B Rabbit mAb Catalog No. A0419 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2503 Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE4B (O95155). Sequence MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAASAPGPSQSLGLNVHNMTPATSPIGASGVAHRSQSSEGVSSLSSSPSNSLETQSQSL Gene ID Swiss Prot Synonyms E4; UFD2; HDNB1; UBOX3; UFD2A Calculated MW 146kDa Observed MW 146kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples SKOV3, K-562, NIH/3T3, Mouse liver, Mouse brain, PC-12, Rat liver, Rat brain Cellular location cytoplasm, nucleus 
Western blot analysis of extracts of various cell lines, using UBE4B antibody (A0419) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE4B (O95155). Isotype IgG