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Размер
- 100 μL
- 200 μL
- 50 μL
ACAA2 Rabbit mAb (A0664)
- Описание
- Характеристики
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Overview
Product name ACAA2 Rabbit mAb Catalog No. A0664 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2533 Background
The encoded protein catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 298-397 of human ACAA2 (P42765). Sequence PAISGALKKAGLSLKDMDLVEVNEAFAPQYLAVERSLDLDISKTNVNGGAIALGHPLGGSGSRITAHLVHELRRRGGKYAVGSACIGGGQGIAVIIQSTA Gene ID Swiss Prot Synonyms DSAEC Calculated MW 42kDa Observed MW 42kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HeLa, HepG2, A-549, Rat heart Cellular location Mitochondrion 
Western blot analysis of extracts of various cell lines, using ACAA2 antibody (A0664) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunofluorescence analysis of HepG2 cells using ACAA2 Rabbit mAb (A0664) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using ACAA2 Rabbit mAb (A0664) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using ACAA2 Rabbit mAb (A0664) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 298-397 of human ACAA2 (P42765). Isotype IgG