быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Active Caspase-3 Rabbit mAb (A11021)
- Описание
- Характеристики
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Overview
Product name Active Caspase-3 Rabbit mAb Catalog No. A11021 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0185 Background
The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CASP3 (P42574). Sequence MENTENSVDSKSIKNLEPKIIHGSESMDSGISLDNSYKMDYPEMGLCIIINNKNFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELM Gene ID Swiss Prot Synonyms CPP32; SCA-1; CPP32B Calculated MW 32kDa Observed MW 17kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples Jurkat+Staurosporine Cellular location Cytoplasm 
Western blot analysis of extracts of Jurkat cells, using Active Caspase-3 Rabbit mAb (A11021) at 1:500 dilution.Jurkat cells were treated by Staurosporine(1 μM) at room temperature for 3 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
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Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CASP3 (P42574). Isotype IgG