быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Wnt16 Rabbit mAb (A20899)
- Описание
- Характеристики
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Overview
Product name Wnt16 Rabbit mAb Catalog No. A20899 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2862 Background
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It contains two transcript variants diverging at the 5' termini. These two variants are proposed to be the products of separate promoters and not to be splice variants from a single promoter. They are differentially expressed in normal tissues, one of which (variant 2) is expressed at significant levels only in the pancreas, whereas another one (variant 1) is expressed more ubiquitously with highest levels in adult kidney, placenta, brain, heart, and spleen.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Wnt16 (Q9UBV4). Sequence MDRAALLGLARLCALWAALLVLFPYGAQGNWMWLGIASFGVPEKLGCANLPLNSRQKELCKRKPYLLPSIREGARLGIQECGSQFRHERWNCMITAAATT Gene ID Swiss Prot Synonyms Calculated MW 41kDa Observed MW 43kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples Raji, 293T, Mouse kidney, Rat brain Cellular location cytoplasm, extracellular region, extracellular space 
Western blot analysis of extracts of various cell lines, using Wnt16 antibody (A20899) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Wnt16 (Q9UBV4). Isotype IgG