быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
USF1 Rabbit mAb (A20903)
- Описание
- Характеристики
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Overview
Product name USF1 Rabbit mAb Catalog No. A20903 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2866 Background
This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been defined on chromosome 21.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 100-200 of human USF1 (P22415). Sequence TSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVVTTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKR Gene ID Swiss Prot Synonyms UEF; FCHL; MLTF; FCHL1; MLTFI; HYPLIP1; bHLHb11 Calculated MW 34kDa Observed MW 36kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, NIH/3T3, Jurkat, K-562, THP-1, Rat spleen Cellular location Nucleus 
Western blot analysis of extracts of various cell lines, using USF1 antibody (A20903) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts of Rat spleen, using USF1 antibody (A20903) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 100-200 of human USF1 (P22415). Isotype IgG