быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Transglutaminase 2 (TGM2) Rabbit mAb (A21184)
- Описание
- Характеристики
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Overview
Product name Transglutaminase 2 (TGM2) Rabbit mAb Catalog No. A21184 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC52843 Background
Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 438-687 of human Transglutaminase 2 (TGM2) (NP_004604.2). Sequence DITHTYKYPEGSSEEREAFTRANHLNKLAEKEETGMAMRIRVGQSMNMGSDFDVFAHITNNTAEEYVCRLLLCARTVSYNGILGPECGTKYLLNLNLEPFSEKSVPLCILYEKYRDCLTESNLIKVRALLVEPVINSYLLAERDLYLENPEIKIRILGEPKQKRKLVAEVSLQNPLPVALEGCTFTVEGAGLTEEQKTVEIPDPVEAGEEVKVRMDLLPLHMGLHKLVVNFESDKLKAVKGFRNVIIGPA Gene ID Swiss Prot Synonyms TGC; tTG; G(h); hTG2; TG(C) Calculated MW 77kDa Observed MW 77kDa Applications
Reactivity Human Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:2000 - 1:10000
- IHC-P 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples A-549, K-562, Hep G2 Cellular location cytosol, endoplasmic reticulum, extracellular exosome, focal adhesion, mitochondrion, nucleus, perinuclear region of cytoplasm, plasma membrane 
Western blot analysis of extracts of various cell lines, using Transglutaminase 2 (TGM2) antibody (A21184) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Transglutaminase 2 (TGM2) Rabbit mAb (A21184) at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon using Transglutaminase 2 (TGM2) Rabbit mAb (A21184) at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human placenta using Transglutaminase 2 (TGM2) Rabbit mAb (A21184) at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Confocal imaging of HeLa cells using Transglutaminase 2 (TGM2) Rabbit mAb (A21184, dilution 1:200)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 438-687 of human Transglutaminase 2 (TGM2) (NP_004604.2). Isotype IgG