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ABflo® 647 Rabbit anti-Human TIM-3/HAVCR2 mAb (A22489)
- Описание
- Характеристики
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Overview
Product name ABflo® 647 Rabbit anti-Human TIM-3/HAVCR2 mAb Catalog No. A22489 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC54204-ABf647 Background
The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 22-200 of human TIM-3/HAVCR2 (NP_116171.3). Sequence SEVEYRAEVGQNAYLPCFYTPAAPGNLVPVCWGKGACPVFECGNVVLRTDERDVNYWTSRYWLNGDFRKGDVSLTIENVTLADSGIYCCRIQIPGIMNDEKFNLKLVIKPAKVTPAPTRQRDFTAAFPRMLTTRGHGPAETQTLGSLPDINLTQISTLANELRDSRLANDLRDSGATIR Gene ID Swiss Prot Synonyms TIM3; CD366; KIM-3; SPTCL; TIMD3; Tim-3; TIMD-3; HAVcr-2 Calculated MW 33kDa Observed MW Refer to figures Applications
Reactivity Human Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location cell surface, early endosome, immunological synapse 
Flow cytometry: 1X10^6 MCF7 cells (negative control, left) and Daudi cells (right) were surface-stained with ABflo® 647 Rabbit anti-Human TIM-3/HAVCR2 mAb (A22489, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells was used as blank control (red line).
Flow cytometry: 1X10^6 Daudi cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human TIM-3/HAVCR2 mAb (A22489, 5 μl/Test, right).
Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 647 Rabbit anti-Human TIM-3/HAVCR2 mAb (A22489, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained Human PBMC was used as blank control (red line).
Flow cytometry: 1X10^6 Human PBMC cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human TIM-3/HAVCR2 mAb (A22489, 5 μl/Test, right).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 22-200 of human TIM-3/HAVCR2 (NP_116171.3). Isotype IgG