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Размер
- 100 μL
- 200 μL
- 50 μL
Xanthine Oxidase (XDH) Rabbit PolymAb® (A22647)
- Описание
- Характеристики
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Overview
Product name Xanthine Oxidase (XDH) Rabbit PolymAb® Catalog No. A22647 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC51278_ARC51279 Background
Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism.Immunogen information
Immunogen A synthetic peptide of human Xanthine Oxidase (XDH). Sequence Email for sequence Gene ID Swiss Prot Synonyms XO; XOR; XAN1 Calculated MW 146kDa Observed MW 150kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples mouse lung, rat lung Cellular location Cytoplasm, Peroxisome, Secreted 
Western blot analysis of extracts from Rat lung, using Xanthine Oxidase (XDH) Rabbit PolymAb® (A22647) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts from Mouse lung, using Xanthine Oxidase (XDH) Rabbit PolymAb® (A22647) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded mouse breast using Xanthine Oxidase (XDH) Rabbit PolymAb® (A22647) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat breast using Xanthine Oxidase (XDH) Rabbit PolymAb® (A22647) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Confocal imaging of Hep G2 cells using Xanthine Oxidase (XDH) Rabbit PolymAb® (A22647, at dilution of 1:50) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide of human Xanthine Oxidase (XDH). Isotype IgG