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Размер
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ABflo® 647 Rabbit anti-Human E-Cadherin/CD324 mAb (A22693)
- Описание
- Характеристики
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Overview
Product name ABflo® 647 Rabbit anti-Human E-Cadherin/CD324 mAb Catalog No. A22693 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC57098-ABf647 Background
This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 155-707 of human E-Cadherin/CD324 mAb(NP_004351.1) Sequence DWVIPPISCP ENEKGPFPKN LVQIKSNKDK EGKVFYSITG QGADTPPVGV FIIERETGWL KVTEPLDRER IATYTLFSHA VSSNGNAVED PMEILITVTD QNDNKPEFTQ EVFKGSVMEG ALPGTSVMEV TATDADDDVN TYNAAIAYTI LSQDPELPDK NMFTINRNTG VISVVTTGLD RESFPTYTLV VQAADLQGEG LSTTATAVIT VTDTNDNPPI FNPTTYKGQV PENEANVVIT TLKVTDADAP NTPAWEAVYT ILNDDGGQFV VTTNPVNNDG ILKTAKGLDF EAKQQYILHV AVTNVVPFEV SLTTSTATVT VDVLDVNEAP IFVPPEKRVE VSEDFGVGQE ITSYTAQEPD TFMEQKITYR IWRDTANWLE INPDTGAIST RAELDREDFE HVKNSTYTAL IIATDNGSPV ATGTGTLLLI LSDVNDNAPI PEPRTIFFCE RNPKPQVINI IDADLPPNTS PFTAELTHGA SANWTIQYND PTQESIILKP KMALEVGDYK INLKLMDNQN KDQVTTLEVS VCDCEGAAGV CRKAQPVEAG LQI Gene ID Swiss Prot Synonyms UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324 Calculated MW 97kDa Observed MW Applications
Reactivity Human Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location Actin cytoskeleton, Adherens junction, Apical junction complex, Cell junction, Cytoplasm, Cytoplasmic side of plasma membrane, Endosome, Extracellular exosome, Extracellular region, Flotillin complex, Glutamatergic synapse, Lateral plasma membrane, Perinuclear region of cytoplasm, Plasma membrane, Trans-Golgi network 
Flow cytometry:1X10^6 K-562 cells (negative control, Left) and A431 cells (right) were surface-stained with ABflo® 647 Rabbit anti-Human E-Cadherin/CD324 mAb (A2269, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 A431 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human E-Cadherin/CD324 mAb (A22693, 5 μl/Test, right).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 155-707 of human E-Cadherin/CD324 mAb(NP_004351.1) Isotype IgG