быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
WTAP Rabbit mAb (A22751)
- Описание
- Характеристики
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Overview
Product name WTAP Rabbit mAb Catalog No. A22751 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC56272 Background
The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 212-355 of human WTAP(NP_004897.2). Sequence DEEVEGMQSTILVLQQQLKETRQQLAQYQQQQSQASAPSTSRTTASEPVEQSEATSKDCSRLTNGPSNGSSSRQRTSGSGFHREGNTTEDDFPSSPGNGNKSSNSSEERTGRGGSGYVNQLSAGYESVDSPTGSENSLT Gene ID Swiss Prot Synonyms Mum2 Calculated MW 44kDa Observed MW 60kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:10000 - 1:50000
- IHC-P 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples HeLa, RAW 264.7, Rat thymus Cellular location Nucleus, Nucleus speckle, nucleoplasm 
Western blot analysis of various lysates, using WTAP antibody (A22751) at 1:40000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded mouse liver using WTAP Rabbit mAb (A22751) at dilution of 1:600 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat testis using WTAP Rabbit mAb (A22751) at dilution of 1:600 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 212-355 of human WTAP(NP_004897.2). Isotype IgG