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ABflo® 488 Rabbit anti-Human B7-H2/CD275 mAb (A23103)
- Описание
- Характеристики
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Overview
Product name ABflo® 488 Rabbit anti-Human B7-H2/CD275 mAb Catalog No. A23103 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC58551-ABf488 Background
Enables identical protein binding activity. Predicted to be involved in T cell receptor signaling pathway and positive regulation of interleukin-4 production. Located in cytoplasmic ribonucleoprotein granule and plasma membrane.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 19-256 of human B7-H2/CD275 (NP_056074.1). Sequence DTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSSLENVDSRYRNRALMSPAGMLRGDFSLRLFNVTPQDEQKFHCLVLSQSLGFQEVLSVEVTLHVAANFSVPVVSAPHSPSQDELTFTCTSINGYPRPNVYWINKTDNSLLDQALQNDTVFLNMRGLYDVVSVLRIARTPSVNIGCCIENVLLQQNLTVGSQTGNDIGERDKITENPVSTGEKNAAT Gene ID Swiss Prot Synonyms B7h; B7H2; GL50; B7-H2; B7RP1; CD275; ICOSL; LICOS; B7RP-1; ICOS-L Calculated MW 20kDa/33kDa34kDa Observed MW Applications
Reactivity Human Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location Cytoplasmic ribonucleoprotein granule, external side of plasma membrane, extracellular exosome, membrane, plasma membran 
Flow cytometry:1X10^6 SH-SY5Y cells (negative control, Left) and Daudi cells (Right) were surface-stained with ABflo® 488 Rabbit anti-Human B7-H2/CD275 mAb(A23103, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells was used as blank control (red line).
Flow cytometry:1X10^6 Daudi cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human B7-H2/CD275 mAb(A23103, 5 μl/Test, right).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 19-256 of human B7-H2/CD275 (NP_056074.1). Isotype IgG