быстрая доставка ~4 недели
Размер
- 100 T
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ABflo® 647 Rabbit anti-Human CD2 mAb (A23117)
- Описание
- Характеристики
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Overview
Product name ABflo® 647 Rabbit anti-Human CD2 mAb Catalog No. A23117 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC59333-ABf647 Background
The protein encoded by this gene is a surface antigen found on all peripheral blood T-cells. The encoded protein interacts with LFA3 (CD58) on antigen presenting cells to optimize immune recognition. A locus control region (LCR) has been found in the 3' flanking sequence of this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 25-209 of human CD2 (NP_001758.2). Sequence KEITNALETWGALGQDINLDIPSFQMSDDIDDIKWEKTSDKKKIAQFRKEKETFKEKDTYKLFKNGTLKIKHLKTDDQDIYKVSIYDTKGKNVLEKIFDLKIQERVSKPKISWTCINTTLTCEVMNGTDPELNLYQDGKHLKLSQRVITHKWTTSLSAKFKCTAGNKVSKESSVEPVSCPEKGLD Gene ID Swiss Prot Synonyms T11; SRBC; LFA-2 Calculated MW 39kDa Observed MW Applications
Reactivity Human Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location Cell membrane, Single-pass type I membrane protein 
Flow cytometry:1X10^6 K-562 cells (negative control, Left) and Jurkat cells (Right) were surface-stained with ABflo® 647 Rabbit anti-Human CD2 mAb(A23117, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 Jurkat cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD2 mAb(A23117, 5 μl/Test, right).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 25-209 of human CD2 (NP_001758.2). Isotype IgG