быстрая доставка ~4 недели
Размер
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ABflo® 488 Rabbit anti-Puromycin mAb (A23130)
- Описание
- Характеристики
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Overview
Product name ABflo® 488 Rabbit anti-Puromycin mAb Catalog No. A23130 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC58626-ABf488 Background
Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that causes premature chain termination during translation taking place in the ribosome. It has a role as a nucleoside antibiotic, an antiinfective agent, an antineoplastic agent, a protein synthesis inhibitor, an antimicrobial agent, an EC 3.4.11.14 (cytosol alanyl aminopeptidase) inhibitor and an EC 3.4.14.2 (dipeptidyl-peptidase II) inhibitor. It is a conjugate base of a puromycin(1+).Puromycin is an antibiotic that prevents bacterial protein translation. It is utilized as a selective agent in laboratory cell cultures. Puromycin is toxic to both prokaryotic and eukaryotic cells, resulting in significant cell death at appropriate doses.Immunogen information
Immunogen Chemical compounds corresponding to Puromycin Sequence Email for sequence Gene ID Swiss Prot Synonyms Puromycin Calculated MW Observed MW Applications
Reactivity Species independent Tested applications FC FC(Intra) Recommended dilution - FC(Intra) 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Positive samples Cellular location 
Flow cytometry:1X10^6 293T cells (negative control, Left) and 293T (Transfection, right) cells were intracellularly-stained with ABflo® 488 Rabbit anti-Puromycin mAb(A23130, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 293T cells (treated with Puromycin) cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Puromycin mAb(A23130, 5 μl/Test, right).
Flow cytometry:1X10^6 RAW 264.7 cells (negative control, Left) and RAW 264.7(Transfection, right) cells were intracellularly-stained with ABflo® 488 Rabbit anti-Puromycin mAb(A23130, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 Raw264.7 cells(treated with Puromycin) cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Puromycin mAb(A23130, 5 μl/Test, right).
Flow cytometry:1X10^6 C6 cells (negative control, Left) and C6(Transfection, right) cells were intracellularly-stained with ABflo® 488 Rabbit anti-Puromycin mAb(A23130, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 C6 cells(treated with Puromycin) cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Puromycin mAb(A23130, 5 μl/Test, right).
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Host species Кролик Antibody type Primary Antibodies Reactivity Независимый от вида Immunogen Chemical compounds corresponding to Puromycin Isotype IgG