![[KD Validated] Bid Rabbit mAb (A23234)](/upload/resize_cache/iblock/b31/340_340_140cd750bba9870f18aada2478b24840a/qz7ir081d03rkjfsbnega0ku24e4lvbq.jpg "[KD Validated] Bid Rabbit mAb (A23234)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KD Validated] Bid Rabbit mAb (A23234)
- Описание
- Характеристики
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Overview
Product name [KD Validated] Bid Rabbit mAb Catalog No. A23234 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC59702 Background
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2, and thus regulate apoptosis. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found.Immunogen information
Immunogen Recombinant protein of human Bid. Sequence Email for sequence Gene ID Swiss Prot Synonyms FP497 Calculated MW 22kDa Observed MW 22kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T Cellular location Cytoplasm, Mitochondrion membrane, Mitochondrion membrane 
Western blot analysis of THP-1, using Bid Rabbit mAb (A23234) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts from wild type(WT) and Bid Rabbit mAb knockdown (KD) 293T(KD) cells, using Bid Rabbit mAb (A23234) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant protein of human Bid. Isotype IgG