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Размер
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ABflo® 647 Rabbit anti-Human Claudin18.2 mAb (A23392)
- Описание
- Характеристики
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Overview
Product name ABflo® 647 Rabbit anti-Human Claudin18.2 mAb Catalog No. A23392 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC60744-ABf647 Background
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human Claudin18.2(NP_001002026.1). Sequence MAVTACQGLGFVVSLIGIAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGAIGLLVSIFALKCIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKIYDGGARTEDEVQSYPSKHDYV Gene ID Swiss Prot Synonyms SFTA5 Calculated MW 27kDa Observed MW Refer to figures Applications
Reactivity Human Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location Bicellular tight junction, lateral plasma membrane, plasma membrane 
Flow cytometry: 1X10^6 CHO(Transfection) cells were surface-stained with ABflo® 647 Rabbit anti-Human Claudin18.2 mAb(A23392, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line).Non-fluorescently stained CHO(Transfection) cells were used as blank control (red line).
Flow cytometry:1X10^6 CHO(Transfection) cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human Claudin18.2 mAb(A23392, 5 μl/Test, right).
Flow cytometry:1X10^6 NUGC4 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human Claudin18.2 mAb(A23392, 5 μl/Test, right).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human Claudin18.2(NP_001002026.1). Isotype IgG