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Overview
Product name ABflo® 594 Rabbit anti-Mouse CD97 mAb Catalog No. A23823 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC62048-ABflo594 Background
Predicted to enable G protein-coupled receptor activity and chondroitin sulfate binding activity. Acts upstream of or within positive regulation of synapse assembly. Located in external side of plasma membrane. Is expressed in several structures, including central nervous system; eye; genitourinary system; gut; and immune system. Human ortholog(s) of this gene implicated in vibratory urticaria. Orthologous to several human genes including ADGRE5 (adhesion G protein-coupled receptor E5).Immunogen information
Immunogen Recombinant protein of mouse CD97. Sequence Email for sequence Gene ID Swiss Prot Synonyms Cd97; TM7LN1 Calculated MW 90KDa Observed MW Applications
Reactivity Mouse Tested applications FC Recommended dilution - FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Flow Cytometry Positive samples Cellular location 
Flow cytometry: 1X10^6 C57BL/6 mouse Splenocytes were surface-stained with ABflo® 594 Rabbit anti-Mouse CD97 mAb(A23823, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (5 μl/Test, blue line).Non-fluorescently stained C57BL/6 mouse Splenocytes cells were used as blank control (red line).

Flow cytometry: 1X10^6 NTERA-2 cells(negative control) were surface-stained with ABflo® 594 Rabbit anti-Mouse CD97 mAb(A23823, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (5 μl/Test, blue line).Non-fluorescently stained NTERA-2 cells were used as blank control (red line).

Flow cytometry:1X10^6 C57BL/6 mouse Splenocytes were surface-stained with ABflo® 594 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 594 Rabbit anti-Mouse CD97 mAb(A23823, 5 μl/Test, right).
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Key applications Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Мышь Immunogen Recombinant protein of mouse CD97. Isotype IgG







