быстрая доставка ~4 недели
Размер
- 100 T
- 200 T
- 500 T
ABflo® 488 Rabbit anti-Human IL5 mAb (A24233)
- Описание
- Характеристики
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Overview
Product name ABflo® 488 Rabbit anti-Human IL5 mAb Catalog No. A24233 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC53304-ABf488 Background
This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to aminoacids 20-134 of human IL5(NP_000870.1). Sequence PTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES Gene ID Swiss Prot Synonyms IL5; EDF; IL-5; TRF; interleukin-5 Calculated MW 15kDa Observed MW Applications
Reactivity Human Tested applications FC FC(Intra) Recommended dilution - FC(Intra) 5 μl per 10^6 cells in 100 μl volume
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.Key application Positive samples Cellular location Secreted 
Flow cytometry: 1X10^6 293T cells(negative control, left) and 293T (Transfection, right) were intracellularly-stained with ABflo® 488 Rabbit anti-Human IL5 mAb(A24233, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 293T(Transfection) cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human IL5 mAb(A24233, 5 μl/Test, right).
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Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to aminoacids 20-134 of human IL5(NP_000870.1). Isotype IgG