быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
WDR77 Mouse mAb (A9921)
- Описание
- Характеристики
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Overview
Product name WDR77 Mouse mAb Catalog No. A9921 Host species Mouse Purification method Affinity purification Isotype IgG1, Kappa CloneNo. AMC0495 Background
The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant protein of human WDR77 Sequence Email for sequence Gene ID Swiss Prot Synonyms p44; MEP50; MEP-50; HKMT1069; Nbla10071; p44/Mep50 Calculated MW 37kDa Observed MW 39kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples K-562, HeLa, 293T, Jurkat, HepG2, MCF7, SW480 Cellular location Cytoplasm, Nucleus 
Western blot analysis of extracts of various cell lines, using WDR77 antibody (A9921) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s. -
Key applications Western blotting, Immunofluorescence Host species Мышь Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human WDR77 Isotype IgG1, Kappa