быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Phospho-ALOX5-S271 Rabbit pAb (AP0573)
- Описание
- Характеристики
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Overview
Product name Phospho-ALOX5-S271 Rabbit pAb Catalog No. AP0573 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8, 11, 14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7, 9-trans-11, 14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen A synthetic phosphorylated peptide around S271 of human ALOX5 (NP_000689.1). Sequence QLSLE Gene ID Swiss Prot Synonyms 5-LO; 5LPG; LOG5; 5-LOX Calculated MW 78kDa Observed MW 78kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Positive samples HT-29+EGF, HT-29+PMA Cellular location Cytoplasm, Nucleus matrix, Nucleus membrane, Peripheral membrane protein 
Western blot analysis of extracts of HT-29 cells, using Phospho-ALOX5-S271 antibody (AP0573) at 1:1000 dilution. HT-29 cells were treated by PMA/TPA (200nM).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A synthetic phosphorylated peptide around S271 of human ALOX5 (NP_000689.1). Isotype IgG