быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Phospho-Bcl-2-S70 Rabbit pAb (AP0575)
- Описание
- Характеристики
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Overview
Product name Phospho-Bcl-2-S70 Rabbit pAb Catalog No. AP0575 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.Immunogen information
Immunogen A synthetic phosphorylated peptide around S70 of human Bcl-2 (NP_000624.2). Sequence RTSPL Gene ID Swiss Prot Synonyms Bcl-2; PPP1R50 Calculated MW 26kDa Observed MW 28kDa Applications
Reactivity Human, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples Jurkat, 293 Cellular location Endoplasmic reticulum membrane, Mitochondrion outer membrane, Nucleus membrane, Single-pass membrane protein 
Western blot analysis of extracts of Jurkat and 293 cells, using Phospho-Bcl2 -S70 antibody (AP0575) at 1:1000 dilution. Jurkat cells were treated by serum-starvation overnight. 293 cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Immunofluorescence analysis of HeLa cells using BCL2 antibody (AP0575) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Крыса Immunogen A synthetic phosphorylated peptide around S70 of human Bcl-2 (NP_000624.2). Isotype IgG