быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Phospho-cdc25C-S216 Rabbit pAb (AP0577)
- Описание
- Характеристики
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Overview
Product name Phospho-cdc25C-S216 Rabbit pAb Catalog No. AP0577 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described.Immunogen information
Immunogen A synthetic phosphorylated peptide around S216 of human cdc25C (NP_001781.2). Sequence SPSMP Gene ID Swiss Prot Synonyms CDC25; PPP1R60 Calculated MW 53kDa Observed MW 60kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples HT-29+λ-PP Cellular location Nucleus 
Western blot analysis of HT-29, using Phospho-cdc25C-S216 Rabbit pAb (AP0577) at 1:400 dilution.HT-29 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A synthetic phosphorylated peptide around S216 of human cdc25C (NP_001781.2). Isotype IgG