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Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Phospho-AKT1S1/PRAS40-T246 Rabbit pAb (AP0793)
- Описание
- Характеристики
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Overview
Product name Phospho-AKT1S1/PRAS40-T246 Rabbit pAb Catalog No. AP0793 Host species Rabbit Purification method Affinity purification Isotype IgG Background
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).Immunogen information
Immunogen A synthetic phosphorylated peptide around T246 of human AKT1S1/PRAS40 (NP_001092102.1). Sequence LNTSD Gene ID Swiss Prot Synonyms Lobe; PRAS40 Calculated MW 27kDa Observed MW 40kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa Cellular location Cytoplasm, cytosol 
Western blot analysis of HeLa, using Phospho-AKT1S1/PRAS40-T246 Rabbit pAb (AP0793) at 1:400 dilution.HeLa cells were treated by Insulin (100 nM) at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 45s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A synthetic phosphorylated peptide around T246 of human AKT1S1/PRAS40 (NP_001092102.1). Isotype IgG