быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Phospho-EEF2K-S366 Rabbit pAb (AP0845)
- Описание
- Характеристики
-
Overview
Product name Phospho-EEF2K-S366 Rabbit pAb Catalog No. AP0845 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.Immunogen information
Immunogen A synthetic phosphorylated peptide around S366 of human EEF2K (NP_037434.1). Sequence TLSGS Gene ID Swiss Prot Synonyms eEF-2K; CaMKIII; HSU93850 Calculated MW 82kDa Observed MW 110kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, NIH/3T3, C6 Cellular location cytoplasm, cytosol, dendritic spine, glutamatergic synapse, postsynaptic density Customer validation WB(Homo sapiens)
Western blot analysis of extracts of HeLa cells, using Phospho-EEF2K-S366 antibody (AP0845) at 1:1000 dilution. Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
Western blot analysis of extracts of various cell lines, using Phospho-EEF2K-S366 antibody (AP0845) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour or PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts of HeLa cells, using Phospho-EEF2K-S366 pAb (AP0845) at 1:1000 dilution or EEF2K antibody (A5404).Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic phosphorylated peptide around S366 of human EEF2K (NP_037434.1). Isotype IgG