быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Phospho-ARHGEF2-S886 Rabbit pAb (AP0926)
- Описание
- Характеристики
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Overview
Product name Phospho-ARHGEF2-S886 Rabbit pAb Catalog No. AP0926 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified.Immunogen information
Immunogen A synthetic phosphorylated peptide around S886 of human ARHGEF2 (NP_001155855.1?). Sequence RRSLP Gene ID Swiss Prot Synonyms GEF; Lfc; P40; GEFH1; LFP40; GEF-H1; NEDMHM Calculated MW 112kDa Observed MW 120kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples C6 Cellular location Cell junction, Cell projection, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, cytoskeleton, ruffle membrane, spindle, tight junction 
Western blot analysis of extracts of C6 cells, using Phospho-ARHGEF2-S886 antibody (AP0926) at 1:1000 dilution.C6 were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic phosphorylated peptide around S886 of human ARHGEF2 (NP_001155855.1?). Isotype IgG