быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Pan Phospho-Tyrosine Mouse mAb (AP0973)
- Описание
- Характеристики
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Overview
Product name Pan Phospho-Tyrosine Mouse mAb Catalog No. AP0973 Host species Mouse Purification method Affinity purification Isotype IgG2b, kappa CloneNo. AMC0235 Background
Protein phosphorylation is one of the main key regulatory mechanisms by which extracellular signals are conveyed. Tyrosine kinase activation is impactful in many tumor types, and in fact, tyrosine kinase inhibitors are one of the more useful therapeutics currently in cancer treatment.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence containing phosphorylated Y. Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW Refer to figures Applications
Reactivity Human, Mouse, Rat, Other (Wide Range) Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples Jurkat Cellular location 
Western blot analysis of extracts of Jurkat cells, using Pan Phospho-Tyrosine antibody (AP0973) at 1:1000 dilution.Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunofluorescence analysis of NIH-3T3 cells using Pan Phospho-Tyrosine Mouse mAb (AP0973) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Мышь Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса, Другое (Широкий ассортимент) Immunogen A synthetic peptide corresponding to a sequence containing phosphorylated Y. Isotype IgG2b, kappa