быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Phospho-CDC25A-S76 Rabbit pAb (AP1166)
- Описание
- Характеристики
-
Overview
Product name Phospho-CDC25A-S76 Rabbit pAb Catalog No. AP1166 Host species Rabbit Purification method Affinity purification Isotype IgG Background
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen A synthetic phosphorylated peptide around S76 of human CDC25A (NP_001780.2). Sequence MGSSE Gene ID Swiss Prot Synonyms CDC25A2 Calculated MW 59kDa Observed MW 59kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples NIH/3T3 Cellular location cytoplasm, cytosol, nucleoplasm, nucleus 
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-CDC25A-S76 antibody (AP1166) at 1:1000 dilution.NIH/3T3 cells were treated by Nocodazole (50 ng/ml) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic phosphorylated peptide around S76 of human CDC25A (NP_001780.2). Isotype IgG