быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Phospho-BRD4-S498/S499 Rabbit pAb (AP1179)
- Описание
- Характеристики
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Overview
Product name Phospho-BRD4-S498/S499 Rabbit pAb Catalog No. AP1179 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.Immunogen information
Immunogen A synthetic phosphorylated peptide around S498 & S499 of human BRD4 (NP_490597.1). Sequence SDSSTD Gene ID Swiss Prot Synonyms CAP; MCAP; HUNK1; HUNKI; FSHRG4 Calculated MW 152kDa Observed MW 125kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa Cellular location Chromosome, Nucleus 
Western blot analysis of extracts of HeLa cells, using Phospho-BRD4-S498/S499 antibody (AP1179) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A synthetic phosphorylated peptide around S498 & S499 of human BRD4 (NP_490597.1). Isotype IgG