быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Phospho-AMPKβ1-S182 Rabbit pAb (AP1195)
- Описание
- Характеристики
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Overview
Product name Phospho-AMPKβ1-S182 Rabbit pAb Catalog No. AP1195 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.Immunogen information
Immunogen A synthetic phosphorylated peptide around S182 of human AMPKβ1 (NP_006244.2). Sequence SSSPP Gene ID Swiss Prot Synonyms AMPK; HAMPKb Calculated MW 30kDa Observed MW 38kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples C2C12 Cellular location cytoplasm, cytosol, nucleoplasm, nucleus 
Western blot analysis of extracts of C2C12 cells, using Phospho-AMPKβ1-S182 antibody (AP1195) at 1:1000 dilution.C2C12 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded mouse testis using Phospho-AMPKβ1-S182 Rabbit pAb (AP1195) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic phosphorylated peptide around S182 of human AMPKβ1 (NP_006244.2). Isotype IgG