быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Phospho-c-Jun-S63 Rabbit pAb (AP1190)
- Описание
- Характеристики
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Overview
Product name Phospho-c-Jun-S63 Rabbit pAb Catalog No. AP1190 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.Immunogen information
Immunogen A synthetic phosphorylated peptide around S63 of human Jun (NP_002219.1). Sequence LTSPD Gene ID Swiss Prot Synonyms AP1; p39; AP-1; cJUN; c-Jun Calculated MW 36kDa Observed MW 44kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples 293T Cellular location Nucleus 
Western blot analysis of extracts of 293T cells, using Phospho-c-Jun-S63 antibody (AP1190) at 1:1000 dilution.293T cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded rat brain using Phospho-c-Jun-S63 Rabbit pAb (AP1190) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human breast cancer using Phospho-c-Jun-S63 Rabbit pAb (AP1190) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse brain using Phospho-c-Jun-S63 Rabbit pAb (AP1190) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic phosphorylated peptide around S63 of human Jun (NP_002219.1). Isotype IgG