быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Phospho-Beclin 1-Ser15 Rabbit pAb (AP1252)
- Описание
- Характеристики
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Overview
Product name Phospho-Beclin 1-Ser15 Rabbit pAb Catalog No. AP1252 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.Immunogen information
Immunogen A synthetic phosphorylated peptide around S15 of human Beclin 1 (NP_003757.1). Sequence QVSFV Gene ID Swiss Prot Synonyms ATG6; VPS30; beclin1 Calculated MW 52kDa Observed MW 60kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Positive samples NIH/3T3, C6 Cellular location Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus, Mitochondrion, Mitochondrion membrane, Nucleus, Peripheral membrane protein, autophagosome, trans-Golgi network membrane 
Western blot analysis of extracts of various cell lines, using Phospho-Beclin 1-Ser15 antibody (AP1252) at 1:500 dilution.NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic phosphorylated peptide around S15 of human Beclin 1 (NP_003757.1). Isotype IgG