быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Phospho-CFL1-Y139 Rabbit pAb (AP0330)
- Описание
- Характеристики
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Overview
Product name Phospho-CFL1-Y139 Rabbit pAb Catalog No. AP0330 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.Immunogen information
Immunogen A synthetic phosphorylated peptide around Y139 of human Phospho-CFL1-Y139 (NP_005498.1). Sequence ANCYE Gene ID Swiss Prot Synonyms CFL; cofilin; HEL-S-15 Calculated MW 19kDa Observed MW Refer to figures Applications
Reactivity Human, Mouse, Rat Tested applications IHC-P Recommended dilution - IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Immunohistochemistry Positive samples Cellular location Cell projection, Cytoplasm, Cytoplasmic side, Nucleus matrix, Peripheral membrane protein, cytoskeleton, lamellipodium membrane, ruffle membrane 
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Phospho-CFL1-Y139 Rabbit pAb (AP0330) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Phospho-CFL1-Y139 Rabbit pAb (AP0330) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat kidney using Phospho-CFL1-Y139 Rabbit pAb (AP0330) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic phosphorylated peptide around Y139 of human Phospho-CFL1-Y139 (NP_005498.1). Isotype IgG