быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Phospho-α-Synuclein-Y133 Rabbit pAb (AP0451)
- Описание
- Характеристики
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Overview
Product name Phospho-α-Synuclein-Y133 Rabbit pAb Catalog No. AP0451 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene.Immunogen information
Immunogen A phospho specific peptide corresponding to residues surrounding Y133 of human alpha-Synuclein Sequence Email for sequence Gene ID Swiss Prot Synonyms PD1; NACP; PARK1; PARK4 Calculated MW 14kDa Observed MW 20kDa Applications
Reactivity Human, Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Positive samples Jurkat, NIH/3T3 Cellular location Cell junction, Cytoplasm, Membrane, Nucleus, Secreted, cytosol, synapse 
Western blot analysis of extracts of various cell lines, using Phospho-α-Synuclein-Y133 pAb (AP0451) at 1:1000 dilution.Jurkat and NIH/3T3 cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen A phospho specific peptide corresponding to residues surrounding Y133 of human alpha-Synuclein Isotype IgG