быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Phospho-CDK4-T172 Rabbit pAb (AP0593)
- Описание
- Характеристики
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Overview
Product name Phospho-CDK4-T172 Rabbit pAb Catalog No. AP0593 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.Immunogen information
Immunogen A synthetic phosphorylated peptide around T172 of human CDK4 (NP_000066.1). Sequence ALTPV Gene ID Swiss Prot Synonyms CMM3; PSK-J3 Calculated MW 34kDa Observed MW 33kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Positive samples NIH/3T3 Cellular location Cytoplasm, Membrane, Nucleus Customer validation WB(Homo sapiens, Capra hircus, Mus musculus)
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-CDK4-T172 pAb (AP0593) at 1:1000 dilution or CDK4 antibody (A0366).NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic phosphorylated peptide around T172 of human CDK4 (NP_000066.1). Isotype IgG