быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Phospho-CDK9-T186 Rabbit pAb (AP0810)
- Описание
- Характеристики
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Overview
Product name Phospho-CDK9-T186 Rabbit pAb Catalog No. AP0810 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.Immunogen information
Immunogen A synthetic phosphorylated peptide around T186 of human CDK9 (NP_001252.1). Sequence RYTNR Gene ID Swiss Prot Synonyms TAK; C-2k; CTK1; CDC2L4; PITALRE Calculated MW 43kDa Observed MW 43kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples NIH/3T3 Cellular location Cytoplasm, Nucleus, PML body Customer validation WB(Human fibrosarcoma cells, HeLa cells, Mus musculus)
Western blot analysis of extracts of NIH/3T3, using Phospho-CDK9-T186 antibody (AP0810) at 1:1000 dilution.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
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Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic phosphorylated peptide around T186 of human CDK9 (NP_001252.1). Isotype IgG