быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Phospho-AP2M1-T156 Rabbit pAb (AP0823)
- Описание
- Характеристики
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Overview
Product name Phospho-AP2M1-T156 Rabbit pAb Catalog No. AP0823 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen A synthetic phosphorylated peptide around T156 of human AP2M1 (NP_001020376.1). Sequence TGQIG Gene ID Swiss Prot Synonyms mu2; AP50; MRD60; CLAPM1 Calculated MW 50kDa Observed MW 50kDa Applications
Reactivity Human, Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T, NIH/3T3 Cellular location Cell membrane, Cytoplasmic side, Membrane, Peripheral membrane protein, coated pit Customer validation WB(Homo sapiens)
Western blot analysis of extracts of 293T and NIH/3T3 cells, using Phospho-AP2M1-T156 antibody (AP0823) at 1:1000 dilution. 293T cells were treated by Calyculin A for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by Hydrogen Peroxide (2nM) for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen A synthetic phosphorylated peptide around T156 of human AP2M1 (NP_001020376.1). Isotype IgG