быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0883)
- Описание
- Характеристики
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Overview
Product name Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb Catalog No. AP0883 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.Immunogen information
Immunogen A synthetic phosphorylated peptide around T183 of human PRKAA1PRKAA1 (NP_006242.5). Sequence LRTSC Gene ID Swiss Prot Synonyms AMPKa1/AMPKa2 Calculated MW 64kDa/65kDa/62kDa Observed MW 62kDa Applications
Reactivity Human, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T, C6 Cellular location apical plasma membrane, cytoplasm, cytosol, nuclear speck, nucleoplasm, nucleus Customer validation WB(Rattus norvegicus, Mus musculus)
Western blot analysis of extracts of various cell lines, using Phospho-AMPKa1-T183/AMPKa2-T172 antibody (AP0883) at 1:1000 dilution.293T and C6 cells were treated by ATP(5 mM) at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Крыса Immunogen A synthetic phosphorylated peptide around T183 of human PRKAA1PRKAA1 (NP_006242.5). Isotype IgG