быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Phospho-c-Myc-T58/S62 Rabbit mAb (AP0988)
- Описание
- Характеристики
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Overview
Product name Phospho-c-Myc-T58/S62 Rabbit mAb Catalog No. AP0988 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1532 Background
This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.Immunogen information
Immunogen A synthetic phosphorylated peptide around T58 & S62 of human c-Myc (P01106). Sequence TPPLS Gene ID Swiss Prot Synonyms MRTL; MYCC; c-Myc; bHLHe39 Calculated MW 51kDa Observed MW 51kDa Applications
Reactivity Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples C6 Cellular location Nucleus, nucleolus, nucleoplasm 
Western blot analysis of extracts of C6 cells, using Phospho-c-Myc-T58/S62 Rabbit mAb (AP0988) at 1:1000 dilution.C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Крыса Immunogen A synthetic phosphorylated peptide around T58 & S62 of human c-Myc (P01106). Isotype IgG