быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Phospho-EGFR-Y1068 Rabbit mAb (AP0994)
- Описание
- Характеристики
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Overview
Product name Phospho-EGFR-Y1068 Rabbit mAb Catalog No. AP0994 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1538 Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).Immunogen information
Immunogen A phospho specific peptide corresponding to residues surrounding Y1068 of human EGFR (P00533). Sequence Email for sequence Gene ID Swiss Prot Synonyms ERBB; ERRP; HER1; mENA; ERBB1; PIG61; NISBD2 Calculated MW 134kDa Observed MW 175kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples BxPC-3+EGF Cellular location Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein 
Western blot analysis of extracts of BxPC-3 cells, using Phospho-EGFR-Y1068 Rabbit mAb (AP0994) at 1:1000 dilution.BxPC-3 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A phospho specific peptide corresponding to residues surrounding Y1068 of human EGFR (P00533). Isotype IgG