быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Phospho-Cdk1/2-T14 Rabbit mAb (AP1001)
- Описание
- Характеристики
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Overview
Product name Phospho-Cdk1/2-T14 Rabbit mAb Catalog No. AP1001 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1546 Background
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]Immunogen information
Immunogen A synthetic phosphorylated peptide around T14 of human Cdk1/2CDK1 (P06493). Sequence EGTYG Gene ID Swiss Prot Synonyms CDC2; CDC28A; P34CDC2 Calculated MW 34kDa Observed MW 34kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, HeLa+Hydroxyurea, HeLa+Nocodazole, NIH/3T3, NIH/3T3+Hydroxyurea, NIH/3T3+Nocodazole, C6, C6+Hydroxyurea, C6+Nocodazole Cellular location Centrosome, Cytoplasm, Cytosol, Extracellular exosome, Mitochondrial matrix, Mitochondrion, Mitotic spindle, Nucleoplasm, Nucleus, Spindle microtubule 
Western blot analysis of extracts of various cell lines, using Phospho-Cdk1/2-T14 Rabbit mAb (AP1001) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by Hydroxyurea (4 Mm) at 37℃ for 20 hours or treated by Nocodazole (100 ng/mL) at 37℃ for 24 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic phosphorylated peptide around T14 of human Cdk1/2CDK1 (P06493). Isotype IgG