быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Phospho-AMPKα1-S496 Rabbit mAb (AP1002)
- Описание
- Характеристики
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Overview
Product name Phospho-AMPKα1-S496 Rabbit mAb Catalog No. AP1002 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1547 Background
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.Immunogen information
Immunogen A synthetic phosphorylated peptide around S496 of human AMPKα1 (Q13131). Sequence SGSVS Gene ID Swiss Prot Synonyms AMPK; AMPKa1; AMPK alpha 1 Calculated MW 64kDa Observed MW 64kDa Applications
Reactivity Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples C2C12+oligomycin Cellular location Cytoplasm, Nucleus Customer validation WB(Mus musculus, Homo sapiens, Rattus norvegicus, Homo sapiens，Mus musculus, Gallus gallus, Sus scrofa)
Western blot analysis of extracts of C2C12 cells, using Phospho-AMPKα1-S496 Rabbit mAb (AP1002) at 1:1000 dilution.C2C12 cells were treated by oligomycin (0.5 μM) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Мышь Immunogen A synthetic phosphorylated peptide around S496 of human AMPKα1 (Q13131). Isotype IgG