быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Phospho-c-Jun-T91 Rabbit mAb (AP1003)
- Описание
- Характеристики
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Overview
Product name Phospho-c-Jun-T91 Rabbit mAb Catalog No. AP1003 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1548 Background
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.Immunogen information
Immunogen A synthetic phosphorylated peptide around T91 of human c-Jun (P05412). Sequence TTTPT Gene ID Swiss Prot Synonyms AP1; p39; AP-1; cJUN; c-Jun Calculated MW 36kDa Observed MW 43kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:2000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples NIH/3T3+UV, NIH/3T3+Anisomycin, 293T+Anisomycin Cellular location Nucleus 
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-c-Jun-T91 Rabbit mAb (AP1003) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes or treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts of 293T cells, using Phospho-c-Jun-T91 Rabbit mAb (AP1003) at 1:1000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded human breast using Phospho-c-Jun-T91 Rabbit mAb (AP1003) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic phosphorylated peptide around T91 of human c-Jun (P05412). Isotype IgG