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Размер
- 100 μL
- 200 μL
- 50 μL
Phospho-Bad-S112 Rabbit mAb (AP1010)
- Описание
- Характеристики
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Overview
Product name Phospho-Bad-S112 Rabbit mAb Catalog No. AP1010 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1555 Background
The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL (B-cell lymphoma-extra large) and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.Immunogen information
Immunogen A synthetic phosphorylated peptide around S112 of human BAD (Q92934). Sequence Email for sequence Gene ID Swiss Prot Synonyms BBC2; BCL2L8 Calculated MW 18kDa Observed MW 23kDa Applications
Reactivity Human, Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples NIH/3T3+PMA/TPA, NIH/3T3+Forskolin Cellular location Cytoplasm, Mitochondrion outer membrane 
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Bad-S112 Rabbit mAb (AP1010) at 1:1000 dilution.NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight or treated by Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen A synthetic phosphorylated peptide around S112 of human BAD (Q92934). Isotype IgG