быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Phospho-ATF2-T71 Rabbit mAb (AP1051)
- Описание
- Характеристики
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Overview
Product name Phospho-ATF2-T71 Rabbit mAb Catalog No. AP1051 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1598 Background
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this geneImmunogen information
Immunogen A synthetic phosphorylated peptide around T71 of human ATF2 (P15336). Sequence TPTPT Gene ID Swiss Prot Synonyms HB16; CREB2; TREB7; CREB-2; CRE-BP1 Calculated MW 55kDa Observed MW 70kDa Applications
Reactivity Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples NIH/3T3+Anisomycin, NIH/3T3 Cellular location Cytoplasm, Mitochondrion outer membrane, Nucleus Customer validation WB(Mus musculus)
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ATF2-T71 Rabbit mAb (AP1051) at 1:1000 dilution.NIH/3T3 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Мышь Immunogen A synthetic phosphorylated peptide around T71 of human ATF2 (P15336). Isotype IgG